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KMID : 0525720170220030149
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Journal of Chitin and Chitosan
2017 Volume.22 No. 3 p.149 ~ p.155
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Production of N-acetyl-glucosamine by Chitinase from Serratia marcescens PRNK-1 Using Chitin Obtained from Edible Tenebrio molitor Larva
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Seo Dong-Jun
Moon Chae-Yeong
Song Yong-Su
Choi Seung-Hee
Han Yeon-Soo
Jo Yong-Hun
Noh Mi-Young
Jung Woo-Jin
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Abstract
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In this study, crab shell chitin and Tenebrio molitor larva (Mealworm) chitin were characterized by X-ray diffraction and FTIR analysis. From the X-ray diffraction results, α-form chitin was observed four crystalline reflections, shown at 9.4°, 19.3°, 20.8°, and 23.3° in crab shell chitin, and at 9.44°, 19.3°, 20.7°, and 23.3° in mealworm chitin by crystalline structure. From the FT-IR spectra results, α-form chitin showed doublet at amide I band in both crab shell chitin and mealworm chitin. Manufacturing process of colloidal chitin from mealworm was conducted with acid, alkali, and decoloration and then adjusted at pH 6~7 with 70% ethanol. Degradation pattern of colloidal chitin from crab shell and mealworm was investigated after reaction of chitinase-producing bacterium Serratia marcescens PRNK-1 by TLC and HPLC. Production of N-acetyl-glucosamine showed rapidly at 3 hr after reaction with crab shell and mealworm colloidal chitin and crude enzyme of S. marcescens PRNK-1 on TLC plates. N-acetyl-glucosamine was produced by 5,041.3 ppm and 5,319.8 ppm at 3 days in crab shell colloidal chitin and in mealworm colloidal chitin, respectively, after reaction with crude enzyme of S. marcescens PRNK-1. Our results indicate that the colloidal chitin obtained from mealworm could be used as useful industrial resources at production of N-acetyl-glucosamine.
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KEYWORD
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Mealworm, Crab shell, Serratia marcescens, N-Acetyl-glucosamine, Colloidal chitin
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